Are there special media requirements in terms of nutrients, supplements, etc. necessary if you are using CHO cells for manufacturing antibodies using perfusion mode?

Question

Accepted Answer

You need to be aware that different nutrients are used at different rates and what is overly utilized is cell specific.  With CHO cells glutamic acid, aspartic acid and cystene are self limiting and need to be part of a perfusion medium.  Glutamine or glutamine dipeptide needs to be added at about 0.5 mM in the medium and with CHO cells the glucose concentration should be  monitored and adjusted daily to 1.0 G/L.

Answer

If you are trying to remove or reduce serum in mesenchymal cells, what are some key components – supplements, growth factors, etc. that you need to include in your classic media.

What do you recommend for an good productive hybridoma media, but also cost effective. Would you make your own using classical or buy off the shelf?

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