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Job Title: Research scientist
For best results you probably want to grow your cells in suspension and in a CD-medium. The various CD media are cell type specific as different cells utilize the amino acids at different rates. You also have to decide if you are going with fed-batch or perfusion. You would be better off buying a specialty medium rather then making your own. All of the CD formulations are about the same qualitatively, but differ quantitatively. They are also complicated in design requiring extensive labor. A typical CD medium would have about 25 trace elements in it. Whether you use fed-batch or perfusion you need to add back the self-limiting amino acids or feed with complete media. With the NSO hybridomas the self-limiting amino acids are cysteine, leucine, methionine, tyrosine and valine (and of course glutamine). To control ammonia and lactate accumulation you need to try to maintain glutamine at 2 mM (to do the same thing with CHO cells you would maintain glucose at 1.0 gm/L). As far as what hybridoma medium to choose, I have only used Hybridoma SFM and later CD-Hybridoma from GIBCO as that is where I used to work.