Are you aware of any components other than dextran sulfate which interfere with transfection?
This question is part of the following Ask The Expert session:
Maximizing Transient Protein Production
Answered by:
Answer
It has been shown that lipids, including cholesterol, in the medium can significantly impact transfection efficiency. That’s why screening and selection of the basal growth medium is important to achieve good transfection efficiency. Besides chemical components, healthy, low passage cells are also critical for maximizing transfection results.
This post is sponsored by MaxCyte.