Which is best in your opinion, transfecting CHO as adherent or in suspension?
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Maximizing Transient Protein Production
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We find similar transfection efficiencies and cell viabilities for suspension and adherent CHO cells. When possible, however, I would recommend the use of suspension CHO cells. It is far easier to conduct the transfection using a chemically-defined production process (animal component free process) for suspension cells as well as allows significantly easier scalability.
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