Can I transfer cells that have been stored at -80 to liquid nitrogen directly or do I have to take interim steps? This question is part of the following Ask The Expert session: The Dos and Don’ts of Cryopreservation Answered by: Dr. Kyung-A Song Company: Corning Job Title: Scientific Support Specialist Answer In general, cryovials can be transferred from -80°C to -196°C directly for long-term storage. Previous Question: Is there a best way to prepare tissue samples for freezing if they will be heavily imaged post thaw? Also do you have recommendations for thaw when the tissue sample will be imaged? This is the last question in the session. This post is sponsored by Corning.