Can I use insulated cardboard or polystyrene foam boxes as a freezing chamber in freezers?
This question is part of the following Ask The Expert session:
The Dos and Don’ts of Cryopreservation
Job Title: Scientific Support Specialist
These homemade devices may work with many cell lines. However, they don’t always give controlled, reproducible, or uniform cooling. As a result, there may be serious differences in viability among the vials upon thawing. Therefore, we do not recommend using insulated cardboard or polystyrene foam for your culture.