Can you describe the ideal cell culture conditions for the best transfection results and does serum in the media have any impact on transfection success?

Answer

The ideal cells are between passages 5-20 after thawing and growing in log phase. Prior to transfection, cell viability should be greater than 90% and 70~80% confluent. For transfection, we recommend using Opti-MEM (serum-free) for lipid/payload complex formation and culture medium supplemented with 10% serum. Lower amount of serum (<10%) works as well but post-transfection (4-24 hr) media change with complete growth media is recommended.