Life Technologies is now part of Thermo Fisher Scientific.
(NYSE: TMO) is the world leader in serving science, with revenues of $17 billion and approximately 50,000 employees in 50 countries. Our mission is to enable our customers to make the world healthier, cleaner and safer. We help our customers accelerate life sciences research, solve complex analytical challenges, improve patient diagnostics and increase laboratory productivity. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services – we offer an unmatched combination of innovative technologies, purchasing convenience and comprehensive support. For more information, please visit www.thermofisher.com.
Job Title: Sr. Product Manager
Job Title: Director of Product Management & Synthetic Biology
Job Title: Customer Training Manager
Job Title: Staff Scientist
Cell Culture Basics – Avoiding contamination, cell growth and passaging, and proper storage techniques
Introduction Sometimes it’s good to get back to basics. This approach can be especially helpful in cell culture particularly in troubleshooting any culture problems. Do you have questions about aseptic techniques, contamination or the use of ... Continued
Meet the Cells – Get your questions answered about some popular cell lines.
Introduction Do you have questions about stem cells, CHO cells, vero cells, Jurkat cells or HUVEC cells? If so, now is the time to get some answers. Whether its troubleshooting problems in your culture, understanding nutritional ... Continued
Got mRNA? Solve DNA transfection issues with mRNA transfection.
Introduction Typically, DNA enters the nucleus when cells divide because cell division creates small nuclear pores. In non-dividing primary cells DNA doesn’t enter the nucleus making these cells very hard-to-transfect. If DNA entry is a bottleneck, ... Continued
Choosing the Right Genome Editing Technology for your Application and Research – Full Transcript
Question: What tactics can you use to reduce off target effects when using CRISPR? See design strategy and target specificity detail on: The Answer: To answer this question, please see design strategy and target specificity detail on: http://www.lifetechnologies.com/content/dam/LifeTech/global/life-sciences/synthetic-biology/pdfs/0913/CRISPR%20Technical%20Product%20Bulletin%20(Global)_FLR.pdf Question: Can ... Continued
Choosing the Right Genome Editing Technology for your Application and Research
Introduction Genome editing —precise, site-specific DNA modification —can now be achieved through the use of chimeric protein constructs that consist of a sequence-specific binding protein linked to a non-specific endonuclease that cleaves DNA a predictable distance ... Continued
Serum, Methods and Considerations for Use
Introduction The use of serum for the culture of cells may be a century old, but still a mainstay in cell culture applications. It is the foundation for many research protocols. There are many aspects to ... Continued
Transfection Optimization for Improved Efficiency and Performance
Introduction Transfection is a common, yet sophisticated method that is frequently used to artificially deliver nucleic acids (DNA or RNA) into cells for a variety of applications. To efficiently introduce nucleic acids to the cell, a ... Continued
Fluorescent Cell Testing Measures – Assessing cell proliferation and cell health using flow cytometry and imaging platforms
Introduction Cell Proliferation assays are an important set of fluorescence based tests that can monitor cell health, cell division, and cell proliferation using a variety of techniques involving flow cytometry and imaging platforms. From DNA content ... Continued
Fluorescence detection in living cells and ways to improve your image
Introduction Fluorescence imaging of living cells can provide important data regarding the function and localization of proteins and other bio-molecules within a cell or tissue. These images give insight into fundamentally important biological processes and improve ... Continued
Transfection and Selection
Introduction Transfection is often used to transfer an expression plasmid into an animal cell with protein expression as the goal. There are multiple methods to accomplish this and many opportunities to optimize the process. Do you ... Continued
Antibiotics in Cell Culture – What types are available and when and how to utilize them
Introduction Antibiotics are often chemicals and sometimes polypeptides that affect the ability of bacteria to grow. Antibiotics come in two categories, bacteriostatic and bactericidal. Bacteriostatic antibiotics don’t kill but inhibit some vital cellular process such as ... Continued
Matrices for Cell Culture – How to develop healthy attachment
Introduction According to the dictionary, there are many definitions for the word matrix. From my point of view the following three pertain to cell culture; 1) a substance in which something is embedded or enclosed; 2) ... Continued
Monoclonal Antibody Production and the Culturing of Mouse Hybridoma Cells.
Introduction The production and maintenance of a hybridoma cell begins with the fusion of a specific antibody producing B cell, to a cancer B cell called a myeloma, which does not produce an antibody by itself. ... Continued
All About CHO – Everything from the basics to troubleshooting
Introduction In the years since CHO based biopharmaceutical manufacturing began in the 1980’s, the technology has come a long way. Despite continual improvements over the years, including advances in equipment, cell line development practices, and media ... Continued
Introduction Most of us want to be serum-free or move in this direction, but transitioning to a serum-free media can be a daunting task. Some are struggling with how to plan their transition to serum-free conditions. ... Continued
Life Technologies’ Cell Culture App for iPhone or iPad
The app allows you to record cell culture data and store experiment information on your iphone or ipad in the lab as you count cells, perform cell culture calculations and generate growth curves. Then when you return to your desk, export all the information to your computer. ...Continued