Can you explain how you extract the LIF from the rice.
This question is part of the following Ask The Expert session:
Expansion of therapeutic stem cells
Job Title: Cell Culture Scientist
Although the exact extraction conditions for the rhLIF is proprietary, the link http://www.ventria.com/about-us/our-technology explains the entire expression and purification process. Typically with any ExpressTec-derived protein, a simple aqueous extraction is sufficient to remove the protein from the rice grain for subsequent chromatography.