Do you recommend using EB or monolayer culture in this application?

Answer

IntestiCult™ Organoid Growth Medium (Mouse) is optimized to provide an environment that supports the growth of intestinal stem cells located at the base of the intestinal crypts or dissociated into single cells. We recommend using primary crypts derived from the small intestine as the medium allows these crypts to grow into 3D multicellular structures, known as organoids. We have not tested the growth of embryoid bodies or embryonic stem cells cultured in a monolayer with this medium.