I accidentally forgot to passage my organoid culture for over a week. Is it still good or do I have to start over?
This question is part of the following Ask The Expert session:
Using Intestinal Organoids for Intestinal and Stem Cell Research
Company: STEMCELL Technologies Inc.
Job Title: Senior Scientist
Unlike other tissue culture, intestinal organoid culture can often survive for weeks without passaging, so you can continue using it. However, we recommend passaging every 7-10 days to ensure that the organoids remain healthy.