Have you thought about how one can reduce money spent in tissue culture work by reducing the amount of media used?

Answer

One observation that comes up in looking at tissue culture flasks is that not all flasks are designed equally. Flask bottoms that connect to the neck via a slanted slope actually is a non-treated area where cells do no grow, but media covers regardless. So one train of thought is to utilize a flask in culture in which the entire bottom is treated for cell culture, thus maximizing cell growth area and minimizing media wastage. Open for discussion on your thoughts or suggestions.