Hello, I’m working with multiple cancer cell lines. Can I use the same amount of any transfection reagent for different cells lines?

Answer

Great question. The answer is no. Transfection efficiency depends highly on the amount of reagent used per well and maybe very different between reagents. Usually, the protocol that is supplied with the product will provide an optimal range of transfection reagent to use. You can go to www.lifetechnologies.com/us/en/home/life-science/cell-culture.html to get specific, detailed transfection protocol for the cell lines that you are interested in. If you are still not achieving the performance you desire in your particular cell line, further optimization may be necessary.