Hi! What is the best differention medium for hepatogenic differentiation of iPS cells derived from human skin fibroblasts? Thanks.
This question is part of the following Ask The Expert session:
Efficient cell specific differentiation systems for iPSC
Company: Thermo Fisher Scientific, Inc.
Job Title: Director of Research and Development
Several researchers are working on iPSC to hepatic differentiation. Commonly, the first step required is definitive endoderm differentiation. Following the definitive endoderm fate, the cells need to be taken through posterior foregut fate and then towards hepatic. There is no one culture media to accomplish these different steps available today. Currently this process involves the use of specific media systems at each of the step during the differentiation process.
The following reference may be useful to you, however we have not performed this protocol in our labs and cannot comment on reproducibility.
• Highly Efficient Differentiation of Functional Hepatocytes From Human Induced Pluripotent Stem Cells (PMID: 23681950) – http://stemcellstm.alphamedpress.org/content/2/6/409.full.pdf+html