How many passages can I get before I see spontaneous differentiation in my iPSCs?

Answer

Passage number is not a limitation to see spontaneous differentiation for iPSCs. If the critical growth factors that support pluripotency state, such as TGF beta and FGF2 are reduced or completely withdrawn, the cells transition to spontaneous differentiation. Those are the actual limiting factors that determine whether an iPSC cell has to be maintained in PSC state or move to differentiated state.

This is the last question in the session.