How much optimization is required with your system and do you use serum?

Answer

There is no serum used in the system. In fact, both the ExpiCHO medium and ExpiCHO Feed are chemically defined, protein-free, serum-free, animal-origin free formulation produced under GMP guidelines. The amount of optimization of the system depends upon the experience your lab has with transient protein expression. If you have used the Expi293 system in the past, then you already have all of the equipment that you need to run ExpiCHO and the level of training necessary to run the ExpiCHO system would be minimal/none. The ExpiCHO system is designed to work straight out of the box so the only optimization that would need to be done would be to simply ensure that your particular lab equipment is being used within the guidelines provided in the ExpiCHO protocol.