How much starting material is required for production?
This question is part of the following Ask The Expert session:
Plasmid DNA Production for Cell and Gene Therapy
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Company: MilliporeSigma
Job Title: Associate Director, EMEA Segment Development for Cell and Gene Therapy
Answer
In regards to plasmid production, an optimized fermentation process with cell densities ranging from 40-60 g/L can achieve pDNA yields around 1-2 g/L when a high-copy number plasmid is used.
For viral vector production, 0.5 mg of pDNA is required for transient transfection of viral vector (AAV or lentivirus) per one 1 L bioreactor. 1 L of transfected bioreactor typically yields 1E14 viral genomes for AAV and 3E9 viral genomes for lentivirus.
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