MilliporeSigma, a life science leader, is a business of Merck KGaA, Darmstadt, Germany. We offer products to safely and efficiently develop and manufacture therapeutics. Our products and services include single-use manufacturing, filtration, chromatography and purification, virus reduction, pharma and bio-pharma raw materials, drug delivery compounds, validation services and biopharmaceutical training. With the acquisition of Sigma-Aldrich, we now have the largest offering of products and services for formulations, actives and biotechnology processes.
Chemically modified amino acids enable the evolution of fed-batch processes from multiple feeds to single feed strategies
Cell culture optimization is critical to good biomanufacturing. In particular fed-batch cultures require optimization of feed strategies to ensure that cultures are not becoming nutrient depleted, to extend the growth phase and increase product concentration. There are several growth factors, ... Continued
New chemical modification of L-Tyrosine and L-Cysteine increase solubility and stability and permit single feed strategies
A long-standing challenge in the use of amino acids as part of a feed strategy has been the insolubility of tyrosine and the oxidation of cysteine at neutral pH. This has required the use of alkaline feeds to allow solubility ... Continued
Introduction Introduction of oxygen to cell culture media via sparging is critical for efficient bioreactor operation. Since oxygen is sparingly soluble in culture media, efficient sparging methods are important to ensure cells have enough oxygen for growth and productivity. However, hydrodynamic ... Continued