Silver Sponsor
MilliporeSigma
MilliporeSigma, a life science leader, is a business of Merck KGaA, Darmstadt, Germany. We offer products to safely and efficiently develop and manufacture therapeutics. Our products and services include single-use manufacturing, filtration, chromatography and purification, virus reduction, pharma and bio-pharma raw materials, drug delivery compounds, validation services and biopharmaceutical training. With the acquisition of Sigma-Aldrich, we now have the largest offering of products and services for formulations, actives and biotechnology processes.
Associated Experts
Job Title: Head of Laboratory Functional Chemistry
Job Title: Viral Vector Producing Cell Lines, Gene Editing and Novel Modalities R&D, Process Solutions R&D
Job Title: Process Development Scientist
Job Title: Head of Technical Operations, Viral and Gene Therapy Manufacturing
Job Title: Associate Director, EMEA Segment Development for Cell and Gene Therapy
Job Title: Global Product Manager
Job Title: Vice President, End-to-End Processing Solutions within Process Solutions
Associated Content
Design of Experiment Principles to Optimize Benzonase® Endonuclease in Viral Gene Therapy Applications
Benzonase® endonuclease activity is strongly influenced by several factors and balancing these is a critical step during process development to maximize its benefit in the overall workflow....Continued
Platform Approach to Accelerating Cell Line and Process Development
Improve the efficiency of cell line and process development by employing a platform approach, which includes all materials needed to advance from protein sequence to production cell line....Continued
Ask The Expert – Plasmid DNA Production for Cell and Gene Therapy
For this Ask the Expert Session, we assembled a team of experts to answer questions on plasmid DNA production for cell and gene therapy applications....Continued
Ask the Expert – Viral Vector Manufacturing
Viral vector manufacturing is a critical part of most gene therapy and gene-modified cell therapy production processes. The Lentiviral vector is a commonly used vector for CAR T gene-modified cell therapies and has been key ... Continued
Viral Vector Process Intensification: Stepwise Model for Efficient Scale-up
Overall, the VirusExpress™ 293T Lentiviral Production Platform consistently achieved high functional titers at a large scale, which addresses the industry’s current challenges for lentiviral production process intensification....Continued
GMP-Compliant Benzonase® endonuclease Safety Plus Emprove® Expert Provides Enhanced Risk Mitigation
Benzonase® endonuclease Safety Plus Emprove® Expert is ideally suited for manufacturing of cell and gene therapy agents such as adeno-associated and lentiviruses, oncolytic viruses, and viral vector vaccines....Continued
Efficient DNA Clearance Enables Better, Faster Virus Production
Video highlights the effectiveness of adding Benzonase® endonuclease for downstream virus purification process intensification improving the speed of purification, reducing cost, improving product purity, and yield while also meeting regulatory requirements....Continued
Cool Tool: Fit-for-purpose Cell Therapy Bioprocessing Platform – MilliporeSigma ekko™ System
Cell and gene therapies require innovative processing technologies to meet the demands of moving from research into clinical and commercial manufacturing. Technologies must be designed to increase safety, efficiency and provide cost effective solution. The ... Continued
Balancing Risk, Cost and Speed During Clinical Development While Still Maintaining Quality
In this podcast, we talked with Thierry Cournez Vice President, BioReliance® End-to-End Solutions, MilliporeSigma. We discussed effective ways for emerging biotechs to collect material quickly and cost-effectively for pre-clinical and clinical studies. We also discussed managing the need to move quickly with cost and quality....Continued
Non-Animal Origin cell culture supplements and manufacturing aids for biologics manufacturing
In this podcast, we talked with Dr. Tobias Hertzig, Regulatory Affairs Manager and Dr. Ulrich Tillmann, Global Product Manager, Supplements and Manufacturing Aids both representing Merck KGaA, Darmstadt, Germany. We discussed non-animal origin cell culture media supplements, including regulatory advantages, performance metrics and their use in biologics manufacturing....Continued
Optimizing Fed Batch Culture – Developing New Tools and Methods to Improve Production
In this podcast and accompanying article, we interviewed Dr. Aline Zimmer, Head of R&D, Advanced Cell Culture Technologies at Merck about the importance of optimizing fed batch cell culture for successful biomanufacturing. Aline discussed how her team has developing new tools and methods for optimizing fed batch culture and the impact this has had on productivity and product quality....Continued
Chemically modified amino acids enable the evolution of fed-batch processes from multiple feeds to single feed strategies
Cell culture optimization is critical to good biomanufacturing. In particular fed-batch cultures require optimization of feed strategies to ensure that cultures are not becoming nutrient depleted, to extend the growth phase and increase product concentration. ... Continued
New chemical modification of L-Tyrosine and L-Cysteine increase solubility and stability and permit single feed strategies
A long-standing challenge in the use of amino acids as part of a feed strategy has been the insolubility of tyrosine and the oxidation of cysteine at neutral pH. This has required the use of ... Continued
Addressing Shear Stress in Bioreactors
Introduction Introduction of oxygen to cell culture media via sparging is critical for efficient bioreactor operation. Since oxygen is sparingly soluble in culture media, efficient sparging methods are important to ensure cells have enough oxygen for ... Continued