I have been tasked with developing a protocol for identifying and isolating different cell types from a mixed population of neural cells using flow cytometry. I have been working on using cell surface signatures, but it has been challenging. Could you recommend any materials or methods for review?

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Accepted Answer

The Gibco® Neurobiology Protocol handbook provides a comprehensive list of methods and protocols to support neural cell research. The handbook includes protocols with topics including neural cell culture and differentiation, cell analysis, molecular characterization, and transfection. (http://www.lifetechnologies.com/us/en/home/global/forms/gibco-neurobiology-handbook.html)

In addition to marker expression, reagents such as fluorescent probes can be used to study neural health, anatomy and function. See an overview of products used for neural cell health at http://www.lifetechnologies.com/us/en/home/life-science/cell-analysis/neuroscience.html

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I am hoping you can help with a verification issue. We are currently using telomerase to identify any undifferentiated PSCs. We would like to add a second method for verification purposes. Which method do you think is best?

In my lab we work with cord blood and we are looking for the fastest, simplest way to take a mixed population of cells from cord blood and identify, isolate and characterize the cells. Do you have any recommendations?

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