I have been tasked with developing a protocol for identifying and isolating different cell types from a mixed population of neural cells using flow cytometry. I have been working on using cell surface signatures, but it has been challenging. Could you recommend any materials or methods for review?

Answer

The Gibco® Neurobiology Protocol handbook provides a comprehensive list of methods and protocols to support neural cell research. The handbook includes protocols with topics including neural cell culture and differentiation, cell analysis, molecular characterization, and transfection. (http://www.lifetechnologies.com/us/en/home/global/forms/gibco-neurobiology-handbook.html)

In addition to marker expression, reagents such as fluorescent probes can be used to study neural health, anatomy and function. See an overview of products used for neural cell health at http://www.lifetechnologies.com/us/en/home/life-science/cell-analysis/neuroscience.html