I have not been able to get MCf7 spheroid formation using low adhesion well plates. Do you have any suggestions? I have used these for other cell lines successfully but not ever with MCF7.
This question is part of the following Ask The Expert session:
Spheroid Culture Advancements – Microplates
Job Title: Senior Scientist
Some adherent cell lines do not form spheroids as well as others. The Corning scientific applications team have noticed that MCF7 cells form loose aggregates when using ultra low adhesion methods alone. One way we have been able to help cells like this successfully form spheroids is to seed cells in the presence of methylcellulose or overlay the cells with Corning® Matrigel® matrix the day after seeding in a round bottom ultra-low attachment vessel.