If you want to express an intracellular protein could you still use a mammalian system? Which cell line?

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Transient Protein Production in Mammalian Cells

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Job Title: Associate Director of Cell Biology

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Absolutely. While insect cells are still used quite a bit for expressing intracellular proteins, HEK293 cells also do a good job of expressing non-secreted proteins.

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We have used transient production for a few projects, but are now looking at expanding our use. What kind of volumes could we reasonably expect if we scaled up and would you recommend HEK or CHO cells for high volumes? Also, if we optimized our system, what is the fastest timeline could we achieve?

I have read that pre-treating cells with DMSO can lead to higher yields, do you find this is true. Is there another yield raising method that does not involve DMSO?

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