If you want to express an intracellular protein could you still use a mammalian system? Which cell line?
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Transient Protein Production in Mammalian Cells
Company: Thermo Fisher Scientific, Inc.
Job Title: Associate Director of Cell Biology
Absolutely. While insect cells are still used quite a bit for expressing intracellular proteins, HEK293 cells also do a good job of expressing non-secreted proteins.