What are the most important factors in promoting cilia development? Are there specific supplements or growth factors included in the PneumaCult-ALI media or added separately that support ciliogenesis?
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Culturing epithelial cells using advanced 3D culture systems for airway modeling
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Several factors contribute to robust and efficient mucociliagenesis, including asymmetric nutrition delivery, airlifting in ALI culture and matrices in sphere culture. In all cases, specialized media such as PneumaCult-ALI is pivotal for robust mucociliagenesis. The PneumaCult media formulations are proprietary, so I am unable to share information about the specific components that support efficient differentiation of airway epithelial cells.
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