What are the most important factors in promoting cilia development? Are there specific supplements or growth factors included in the PneumaCult-ALI media or added separately that support ciliogenesis?

Answer

Several factors contribute to robust and efficient mucociliagenesis, including asymmetric nutrition delivery, airlifting in ALI culture and matrices in sphere culture. In all cases, specialized media such as PneumaCult-ALI is pivotal for robust mucociliagenesis. The PneumaCult media formulations are proprietary, so I am unable to share information about the specific components that support efficient differentiation of airway epithelial cells.