In my lab we are working on producing antibody using hybridoma for both in-vivo studies and also pre-clinical work. We are using shake flask, but now need to produce more volume. I am wondering what system you would suggest for smaller scale production and if you would recommend perfusion culture or not.
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Multiple hybridoma cell lines have been successfully cultivated in WAVE Bioreactor systems. Using different Cellbag bioreactors, cultivation volumes from < 0.5 L to 25 L can be run on the same
instrument. After an initial characterization of the cultivation process in batch mode, a process optimization targeting perfusion is a viable option to increase the volumetric productivity of hybridomacultures.
An example for such a perfusion process can be found in: Tang Y. et al. 2007. Biotechnol. Prog. 23(1):255-264. The study showed a 10-fold increase of the viable cell concentration when switching from batch to perfusion culture. This resulted in a substantially higher volumetric productivity and an overall 7-fold increase in the amount of antibody produced in perfusion culture.
This post is sponsored by Cytiva (formerly GE).