Have you looked at whether the disposable material or the products used to initiate the dissolve effect the expression of surface markers morphology, or pluripotentcy of MSCs


Appropriate surface marker morphology and maintenance of pluripotentcy is certain to be of interest for a user of Corning Dissolvable Microcarriers (DMC) when producing stem cells. Understanding this, we evaluated expected surface markers, post-harvest pluripotentcy, and karyotype of stem cells across a multitude of different conditions: Our studies showed that this is true for both denatured collagen and Synthemax® coatings in serum containing and serum free conditions respectively. These data were generated for spinner flask culture as well as conventional stirred tank bioreactors. With regard to materials or products used to initiate the dissolution of the microcarriers, all of the results referenced were generated after growth and harvest from DMC using the dissolution protocol. One study in particular looked at multiple passages on DMC under two different expansion regimens. The first was bead-to-bead expansion with periodic addition of fresh DMC and media, and the other was a dissolution harvest and reseed approach. This study was performed on Synthemax® coated beads using serum free media and showed that after seven passages the harvested cells from both experimental arms maintained CD73/CD105 at ~95% and CD14/CD45 at <1% while also being able to subsequently differentiate into adipogenic, osteogenic, and chondrogenic cell lineages. Other studies showed similar results but also demonstrated appropriate CD90 and CD34 markers as well as assessment of appropriate karyotype.

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