I am looking for a simpler way to analyze my undifferentiated and differentiating iPSC population for biomarker expression and germ layer specific markers to confirm pluripotency. Any suggestions?
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Using Luminex Technology to Non-Invasively Monitor Stem Cell Differentiation
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Company: Bio-Techne
Job Title: Manager of Product Development for the Stem Cell and Cell Culture Department
Answer
The Proteome Profiler Arrays would be a good option for you. R&D Systems has a Human Pluripotent Stem Cell Array that detects 15 pluripotent and germ layer-specific markers simultaneously. This array requires cell lysates rather than cell culture supernatants, which are used for non-invasive monitoring of stem cells. R&D also offers a number of pluripotent and germ layer staining kits which can be viewed here.
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