Media changes in high throughout plates-possible? If so, what’s the best way to do this?


It is possible as long as aspiration is not too close to Matrigel layer. For example, I add 50 uL of cell suspension to the 96 well plates and try to remove 40uL or so before adding another fresh 50uL. Alternatively, I have also had success simply adding more fresh medium or compounds without aspirating any medium.

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