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There are many reports regarding cryopreservation of hepatocytes from different species like mouse, monkey, dog, rat, and human. According to many literature, the authors used 10-20% of DMSO for cryopreservation of hepatocytes. 10% of DMSO is most common concentration as a minimum of cryoprotectant for hepatocytes. If you are worried about cell viability, you can attempt to add oligosaccharides as a supplement in the freezing media that contains 10% of DMSO(1). The authors observed use of oligosaccharides resulted in greatest improvement in cell viability using trypan blue (TB) exclusion method. In addition, a researcher team in Sweden evaluated cell viability of hepatocytes using STEM-CELLBANKERTM (CB) which is a xeno-free cryopreservation solution containing 10% DMSO, glucose and anhydrous dextrose, comparing standard 12% DMSO-UW medium(2). They observed higher cell viability in CB protocols than DMSO-UW in hepatocytes.