In your study, what method are you using to confirm differentiation? Are you measuring marker expression at all stages and/or function?
This question is part of the following Ask The Expert session:
Using Luminex Technology to Non-Invasively Monitor Stem Cell Differentiation
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Company: Bio-Techne
Job Title: Manager of Product Development for the Stem Cell and Cell Culture Department
Answer
We have used this technology for iPSC-derived hepatocytes and iPSC-derived cardiomyocytes. For hepatocytes, we used our StemXVivo Hepatocyte Differentiation Kit to differentiate, which we have verified for expression of stage-specific markers as well as for function, including albumin secretion, urea secretion, and p450 activity. Hepatocyte verification data can be viewed here. For cardiomyocytes, we used our StemXVivo Cardiomyocyte Differentiation Kit to differentiate, which we have verified for stage specific marker expression, contractility, response to known regulators of cardiomyocyte contractility, and response to cardiotoxic agents. Cardiomyocyte verification data can be viewed here.
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