To follow up on my serum free question – I am having problems with attachment. Cells start out ok but then end up detaching quickly after being in culture.
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I apologize for not being an expert on this topic, and without knowing the exact cell type of stem cells, I was able to look up the following 2 articles that showcase protocols for working with stem cell line expansion in serum free media:
http://www.rndsystems.com/literature_ccm014.aspx
http://www.millipore.com/userguides.nsf/a73664f9f981af8c852569b9005b4eee/9f33a8f6d1bf001b852575060079a208/$FILE/pc1806en00.pdf
Also, have you considered growing the stem cells on a coating to prevent detachment in a serum-free media:
http://www.ncbi.nlm.nih.gov/pubmed/19500111
I hope this points you in the right direction and hopefully we get some additional comments to aid the detachment issue.
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