To follow up on my serum free question – I am having problems with attachment. Cells start out ok but then end up detaching quickly after being in culture.


I apologize for not being an expert on this topic, and without knowing the exact cell type of stem cells, I was able to look up the following 2 articles that showcase protocols for working with stem cell line expansion in serum free media:$FILE/pc1806en00.pdf

Also, have you considered growing the stem cells on a coating to prevent detachment in a serum-free media:

I hope this points you in the right direction and hopefully we get some additional comments to aid the detachment issue.