Using your method, can I confirm that my cells are still pluripotent without damaging them after reprogramming and during expansion.
This question is part of the following Ask The Expert session:
Using Luminex Technology to Non-Invasively Monitor Stem Cell Differentiation
Answered by:
Company: Bio-Techne
Job Title: Manager of Product Development for the Stem Cell and Cell Culture Department
Answer
In order to non-invasively monitor pluripotency, you would first need to determine the signature secretory profile of your pluripotent stem cells. I’d suggest first assessing the secretory profile of your cells with the qualitative Proteome Profile XL Cytokine Array. You can compare your results with the data presented in the webinar, for which we show similar secretory profiles across iPS cell lines. As an alternate, there are pluripotent live-cell staining kits available that allow you to visually confirm pluripotency in live cells using fluorescence without out affecting proliferation or stemness.
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