We are culturing small volumes of hybridoma cells in standard shake flasks. Are there any improved cultureware that you think would improve our process in general?

Answer

Shaking in flasks has come a long way from the ancient glass bottles to flat bottomed flasks to now the conical bottomed TubeSpin Bioreactors. The latter allows for greater gas exchange and internal cyclonic mixing to promote increased cell propagation. Take a look at the two following links and we would suggest sampling the TubeSpins to test it for yourself: Diagram: http://www.tpp.ch/page/downloads/TubeSpin/TubeSpin_vs_Erlenmeyer.jpg ; Poster: http://www.tpp.ch/page/downloads/TubeSpin/2012_ECI_Poster_Monteil.pdf