We are looking for an ultrafiltration solution for our microbioreactor studies. What is the max sample volume you recommend with your systems?
This question is part of the following Ask The Expert session:
Answered by:
Answer
It depends upon what method of ultrafiltration is best suited, however below are the typical volume ranges for our lab based upon the different types of force.
Centrifugation: 100ul to 100ml
Pressurisation: 20ml to 100ml
Tangential Flow Filtration: 100ml to 5000ml
So for example the Sartorius ambr 15 bioreactors produce working culture of 10-15ml, this would work directly with our Vivaspin Turbo 15 or Vivaspin 20 devices. Perhaps the Vivaspin Turbo 4 or Vivaspin 6 devices if some volume is lost after initial clarification.