We are seeing poor dynamic range for our standard curve, could automation improve this?
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How to get the most out of automating your ELISA-like assays, dos and don’ts
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Job Title: Senior Researcher for the High Throughput Antibody Discovery team
Answer
As a general rule, automation will not improve your assay in that fashion. It can reduce variability and tighten %CV, but it is not going to make a mediocre reference molecule better. I would focus my efforts into the assay design/development in this situation. Sensitivity, dynamic range, LOD and LLOQ are functions of the assay design, not the automation. Perhaps there is alternative standard that can be used, or even a surrogate. Perhaps altering some assay conditions can help extend you dynamic range. Don’t be afraid to try some things that you wouldn’t normally do.
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