We are working with mesenchymal stem cells and need to measure their ability to suppress T-cell proliferation. We were outsourcing this, but would like to bring it in-house. Any advice? Is it better to keep outsourcing?


A simple in vitro protocol such as the Mixed Lymphocyte Reaction assay can be used to measure immunosuppression of MSC and AdSCs. An example protocol can be found in this publication: Kruisbeek et al., (2004) Proliferative Assays for T Cell Function. Current Protocols in Immunology 3.12.1-3.12.20. http://www.currentprotocols.com/WileyCDA/CPUnit/refId-im0312.html