What concentration Matrigel should I use for 3D screening?
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Bringing 3D models into a High Throughput Environment
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We recommend using at least 150-200ug/cm2 of Matrigel for 3D applications. The concentration may need to be optimized for your specific applications. Alternatively, you could try out Matrigel Matrix-3D plate which has been optimized for 3D culture and is already coated with unpolymerized Matrigel.