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Job Title: Research scientist
The medium will differ as will the transfection protocol dependent on if you are growing and transfecting adherent cells are cells in suspension. The life Technologies web-site has protocols on it for transfecting either type of cell. I just looked at a couple of them and they were pretty straight forward. I have never tried to transfect cells in suspension, but I know that GIBCO has what they are calling 293 Free Style Expression media, adapted cells and a cationic lipid they are calling 293 fectin. Other companies may have equivalent products. My own experience with transfection is limited. I have only worked with attached cells and the cationic lipids Lipofectamine and Lipofectamine 2000.