What kind of media do you recommend for transient transfection of HEK293 cells? Are there any key components to include or look for in media. Our cells aren’t doing well right now in our current media.

Answer

The medium will differ as will the transfection protocol dependent on if you are growing and transfecting adherent cells are cells in suspension. The life Technologies web-site has protocols on it for transfecting either type of cell. I just looked at a couple of them and they were pretty straight forward. I have never tried to transfect cells in suspension, but I know that GIBCO has what they are calling 293 Free Style Expression media, adapted cells and a cationic lipid they are calling 293 fectin. Other companies may have equivalent products. My own experience with transfection is limited. I have only worked with attached cells and the cationic lipids Lipofectamine and Lipofectamine 2000.