What do you think is the best transfection method for HSCs.

Question

Accepted Answer

Retroviral or lentiviral transduction are the established methods to introduce genes into hematopoietic stem and progenitor cells (HSPCs), and are adequate for research applications. Lentiviral transduction has become the method of choice for gene therapy applications because it results in a higher percentage of successfully transduced HSPCs and is thought to be safer.

Answer

I am seeing doubling time of our HSCs at 36 hours. I would like to decrease that but think that this time is about average. Is this what I should expect or do you have suggestions on how to improve?

I have found some published protocols on generating iPSCs from cord blood, but I am wondering if you have a recommendation on the simplest, most effective way to do this.

Pin It on Pinterest