What do you think is the best transfection method for HSCs.

Answer

Retroviral or lentiviral transduction are the established methods to introduce genes into hematopoietic stem and progenitor cells (HSPCs), and are adequate for research applications. Lentiviral transduction has become the method of choice for gene therapy applications because it results in a higher percentage of successfully transduced HSPCs and is thought to be safer.