What do you think is the best transfection method for HSCs.
This question is part of the following Ask The Expert session:
Hematopoietic Stem & Progenitor Cell Culture
Company: STEMCELL Technologies Inc.
Job Title: Principal Scientist
Retroviral or lentiviral transduction are the established methods to introduce genes into hematopoietic stem and progenitor cells (HSPCs), and are adequate for research applications. Lentiviral transduction has become the method of choice for gene therapy applications because it results in a higher percentage of successfully transduced HSPCs and is thought to be safer.