What do you think is the best way to culture HEK293 cells? Could you use this system?
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Options for Increasing Output and Reducing Footprint in Adherent Cell Culture
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If HEK is in suspension: Grow them in shaken flasks until you reach the desired cell density, then seed the iCELLis bioreactor. From there, grow them until the desired density for transfection and then proceed with addition of the viral vector and transfection reagents.
If HEK is in adherence: Do the same, but replace the shaken flasks of pre-culture by static multitray systems. As before, grow them in shaken flasks until you reach the desired amount of cells, then seed the iCELLis bioreactor. From there, grow them until the desired density for transfection is reached, and then proceed with addition of the viral vector and transfection reagents.
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