What growth factor or factors are key to hepatocyte differentiation from iPSCs?
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Directed Differentiation of Pluripotent Stem Cells
Company: Thermo Fisher Scientific, Inc.
Job Title: Director of Research and Development
To begin with as endoderm, one would critically need high levels of Activin, FGF & BMP, followed by hepatocyte growth factor ( HGF) and Oncostatin in later stages of hepatic differentiation.
For detailed steps and concentration of specific growth factors used in different steps, I recommend the protocol described : Ma et al : Stem Cells Translational Medicine: 2 : 409-419, 2013