What is the best way to identify early on if you have just a group of cells lumped together or an actual spheroid? How quickly should you be able to tell?


Generally, you can make this distinction using phase contrast or brightfield microscopy. If you have a spheroid, you should not see the edges of the individual cells as you would with just a group of cells lumped together. The timing for this is cell type dependent. For example, HT29 cells typically form a spheroid within 1-2 days, but primary human hepatocytes can take up to 7 days to form a spheroid.

This is the last question in the session.

Pin It on Pinterest