What is the most efficient method for developing a stable cell line for lentiviral production?
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Company: MilliporeSigma
Job Title: Head of Technical Operations, Viral and Gene Therapy Manufacturing
Answer
The delivery of viral gene expression constructs into the cells, their respective integration and selection of high producers are relatively difficult and time consuming (again > 1year) at this time in which groups have to screen hundreds if not thousands of clones that produce the LV particle at titers that would be manufacturing and commercially useful. Unlike the use of Mab clones which are driven by constituitive promoters to drive transcription of the IgG genes, several of the viral genes are toxic to the producer cells, therefore requiring the use of inducible promoter systems in order to control the production of these toxic proteins in the cells.
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