What is the seeding density that is recommended for using Lipofectamine 3000 to produce lentivirus?

Answer

Unlike other commonly used transfection reagents that are used in Lentivirus production, Lipofectamine 3000 actually requires a high cell density (cell type: 293T or FT) at the time of transfection. The recommended density should be 95-99% at the time of transfection.