What is your preferred method for confirming differentiation?
This question is part of the following Ask The Expert session:
Directed Differentiation of Pluripotent Stem Cells
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Company: Thermo Fisher Scientific, Inc.
Job Title: Director of Research and Development
Answer
Along the path of differentiation, ensuring early, middle and late stage specific marker expression for different lineages and finally testing the cells for their physiological function – for example, if the cells are being differentiated to dopaminergic neurons, analyzing the dopamine release in these cultures and finally testing the cells in in vivo animal transplants to confirm full differentiation.
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