What techniques can you suggest for improving media solubility for media that is difficult to get into solution?
This question is part of the following Ask The Expert session:
Troubleshooting Cell Culture Media for Bioprocessing
Company: Cytiva (formerly GE)
Job Title: Director of Process Science
There are approaches, such as alternating pH (either acidic or basic) and temperature (mostly warm), that can accelerate/facilitate solubilizing media compounds. However, they don’t necessarily improve solubility (maximum concentration in a saturated solution)issues. Therefore, it is important to also evaluate the composition of your media formulation to assess whether there are components that are at the limit of solubility. Additionally, chelation can be considered for stabilizing the solution of a difficult component. One can also consider exchanging a less soluble component for a biologically equivalent compound with higher solubility to overcome solubility limitations.