Ask the Expert – Hematopoietic Stem & Progenitor Cell Culture

By on November 2, 2015
Ask the Expert

The culture of hematopoietic stem and progenitor cells (HSPCs) is important to assay the quality and functional properties of HSPCs e.g., during the implementation of transplantation or other approaches to treat hematological disorders, such as leukemia. Examples of HSPC culture assays include colony-forming unit (CFU) assays in semi-solid methylcellulose-based media, such as MethoCult™, to identify and quantify HSPCs, and expansion cultures in liquid media, such as StemSpan™, to increase HSPC numbers or generate large numbers of mature blood cells. These culture methods can also used to evaluate the efficacy and toxicity of new drug candidates on hematopoiesis in vitro and to generate target cells for reprogramming to generate induced pluripotent stem cells.

In this Ask the Expert Session, we will discuss challenges and techniques for the culture of HSPCs, including the use of serum-free, defined media and supplements that have been developed by STEMCELL Technologies for HSPC culture applications.

This session is sponsored by STEMCELL Technologies and hosted by Dr. Bert Wognum. Dr. Wognum is the Principal Scientist Ask The Expert STEMCELLfor Hematopoietic & Immunopoietic Products in R&D at STEMCELL Technologies, and currently leads the development of new media and supplements for the expansion, differentiation and detection of HSPCs in culture.

Dr. Wognum obtained his PhD at the University of Amsterdam in the Netherlands and worked as a postdoctoral fellow at the Terry Fox Laboratory in Vancouver, Canada, and the Department of Hematology at Erasmus University in Rotterdam, the Netherlands. He studied the role of hematopoietic growth factors and their receptors in normal hematopoiesis and leukemia and contributed to preclinical studies aimed at improving hematopoietic recovery after cytoreductive therapy and transplantation.

Questions about the culture of of HSPCs?

During this Ask the Expert session, Dr. Bert Wognum will be answering your questions about StemSpan™ media and supplements for serum-free HSPC expansion, along with highlighting recent research regarding the use of small molecules for HSPC expansion.

Topics of discussion may include:

  • The best cell sources for HSPCs
  • Methods to measure HSPC frequencies and properties in culture
  • When to purify HSPCs and how (or when not to) for culture
  • How to use HSPC cultures to measure the hematotoxicity of new drug candidates
  • How to exploit molecular regulators of HSPC function to drive HSPC expansion in culture
  • Methods to generate large numbers of specific blood cells (e.g. erythrocytes and platelets) by promoting HSPC expansion and lineage-specific differentiation.
  • The use of HSPCs and culture-expanded erythroid cells as cell sources for reprogramming to generate pluripotent stem cells
  • Examples of the successful translation of methods to improve outcomes after clinical HSPC transplantation

Please take advantage of the opportunity to ask our expert a question and participate in a discussion on serum-free conditions for the ex vivo expansion of human hematopoietic stem and progenitor cells.

Ask a Question or View Topic

One Comment

  1. Pingback: Culturing Hematopoietic and Progenitor Stem Cells - Key Tips and Tools - Cell Culture Dish

Leave a Reply

Your email address will not be published. Required fields are marked *