What do you recommend for differentiation of blood cell generated iPSCs – EBs or monolayer?
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Using Blood Cells As a Source for Generating Induced Pluripotent Stem Cells
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We typically perform directed differentiation of iPSCs (generated from blood cells) using monolayer cultures.
For examples, please refer to our STEMdiff Neural Induction Medium or STEMdiff Definitive Endoderm kits for our monolayer protocols.
However, should you decide to differentiate using embryoid bodies (EBs), we would recommend using Aggrewell™ plates for reproducible production of uniformly-sized embryoid bodies.
This post is sponsored by STEMCELL Technologies Inc..