All About CHO – Everything from the basics to troubleshooting

Introduction

In the years since CHO based biopharmaceutical manufacturing began in the 1980’s, the technology has come a long way. Despite continual improvements over the years, including advances in equipment, cell line development practices, and media formulation, gains can still be made in CHO based manufacturing. CHO remains, by far, the most popular cell line for best selling biologics on the market today and as such it is imperative that we continue to find ways to improve on product yield, product quality, cost, and a myriad of other manufacturing challenges. If you are looking for ways to improve your CHO cell line or have a challenge to share, please join us for our Ask the Expert session on everything CHO.

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Question 1

What is the difference between all these different types of CHO cells? I read about CHO-K1 and CHO-DG44 all the time.

There are many types of CHO (Chinese Hamster Ovary) cells in use today. It is a bit of a mystery when CHO cells originated but it is thought the line was derived in the 1960’s. Dr. Theodore Puck received a female Chinese Hamster from a laboratory at the Boston Cancer Research Foundation and used the … Continued

Question 2

I would like to know why CHO cells are so special and everyone seems to use them?

Chinese Hamster Ovary cells (CHO) have been around a long time, since the 1960s and as with most of the early-cultured cells they are derived from a rodent. Rodent cells were used to create the first homogeneous cell lines and media formulations. Many events occurred to bring CHO cells to the forefront in biotechnology. CHO … Continued

Question 3

Hi, I am wondering if the CHO genomic sequence known?

This is a good question and the answer is yes! The paper by Xun Xu et al., Nature Biotechnology, Vol 29, No 8, 201, pg 735. reports that a CHO-K1 ancestral cel line was sequenced. There were 24,383 predicted genes. What is very interesting about the paper is that the authors focused on investigating genes … Continued

Question 4

I was wondering if shipping CHO cells requires any permitting or notification.

This is a interesting question and it took a while to dig up the answer but here it it. According to a U.S. Fish and Wildlife Service (FWS) Notice dated December 21, 2012 hamsters are considered wildlife and are not a domesticated species and therefore import and export are regulated by the FWS. So as … Continued