Don’t miss our top Cool Tools of 2020! I have compiled a list of our 10 most popular Cool Tools for 2020 in alphabetical order.

The Addition of Allegro™ STR 500 Enables Seamless Single-Use Bioreactor Scalability from 50 to 2,000 L

Large-scale biomanufacturing frequently relies on single-use bioreactor systems for efficient manufacturing and scale-up. However, one challenge has been predictable scale-up from process scale bioreactors to larger manufacturing volumes. Pall has met this challenge with the Allegro STR bioreactor product line, which includes 50, 200, 1000, 2000 and the new 500 L bioreactor. The consistency in design, engineering and performance across all volumes enables predictable scale-up. In addition, the Allegro STR 500 bioreactor system leverages the usability and process assurance features seen in the entire Allegro STR bioreactor product range…

DASbox Mini Bioreactor System’s small working volumes and advanced control options offer a versatile solution for advanced process development experiments

Process development and optimization is a critical aspect of manufacturing for both cell culture and microbial-based systems. The challenge is often creating conditions in process development that match the conditions found in manufacturing. To fully optimize a process, it is important to be able to run multiple experiments at the same time in order to fully assess the best conditions. A Design of Experiments (DoE) provides an efficient and thorough look at a wide variety of conditions, but in order to fully utilize this tool, you must be able to conduct experiments in parallel. If not, the time and resources required would make DoE not feasible in many situations. If done properly, a good process development strategy can shorten a product’s time to market…

Fit-For-Purpose Cell Therapy Bioprocessing Platform – MilliporeSigma ekko™ System

Cell and gene therapies require innovative processing technologies to meet the demands of moving from research into clinical and commercial manufacturing. Technologies must be designed to increase safety, efficiency and provide cost effective solution. The ekko™ acoustic cell processing system by MilliporeSigma provides a robust platform for cell processing that advances current methods for the concentrate-wash step and a number of other applications. This innovative technology delivers a closed and automated process, thereby increasing efficiency and reducing risk of contamination…

GMP-Compliant Benzonase® Endonuclease Safety Plus Emprove® Expert Provides Enhanced Risk Mitigation

Benzonase^® endonuclease has been used in biopharmaceutical production for DNA removal for over 30 years. It is a genetically engineered endonuclease, from Serratia marcescens, which can completely hydrolyze all forms of DNA and RNA (single-stranded, double-stranded, linear, and circular) into smaller oligonucleotides (<6 kDa) with no base preference. The use of Benzonase^® endonuclease for downstream virus purification improving speed of purification, protects the downstream chromatography and filter devices from fouling, and give better product purity and yield while also meeting regulatory requirements…

Next Generation Bioprocess Controller SciVario® Twin Provides Flexible Solution to Address Current and Future Needs

Process development has always been both a critical, and resource intensive part of biomanufacturing. Thus, the industry is routinely looking for more efficient ways to improve the process. Recently, a modern digital biomanufacturing approach has been gaining acceptance. This approach involves acquiring more process understanding, monitoring and analytics than ever before and is constantly evolving to incorporate new technologies and demands…

Online Platform Makes Customizing Cell Therapy Media Effortless with 400 Publicly Available Formulas and Real Time Pricing Information

Media development for cell therapies can be a time consuming and costly effort, often involving testing of multiple formulas prior to selection. Some opt to select an off-the-shelf media option to save time and streamline development. However, additional customization to these formulations is often difficult because they involve proprietary formulations. In addition, cell therapy companies frequently include media composition as part of their intellectual property portfolio, which isn’t possible with an off-the-shelf formulation. Another option is to create a customized media; this frequently requires combing the literature for publicly available formulas and then customizing to suit an individual process. However there isn’t an easy way to see the effect custom media configurations have on pricing and custom media formulations can frequently take up to six months to manufacture…

Real-Time Protein Titer Measurement Provides Fast, Simple Solution for Process Development and Production

Protein titer data is a critical during process development and manufacturing of protein-based biopharmaceuticals. Speed and accuracy of this data is key in instituting a process analytical technology (PAT) approach, as this allows for monitoring critical process parameters (CPP) and informs real-time decision making. In addition, this type of data when collected during process development enables optimization of cell culture conditions, protein yield, bioreactor run length and harvest time. Collecting this data during a bioreactor run is ideal, but can be time consuming and resource intensive with traditional tools…

The Rebel Analyzer Enables Real-Time Comprehensive Media Analysis in a Benchtop Package

Spent media analysis is a critical part of biotherapeutic process development. The ability to analyze how cell culture components are consumed provides data that speaks to the health and productivity of the culture itself. The data generated allows multiple groups within process development to work together to ensure the best overall process; this includes cell line development, media formulation, process optimization, and purification. While process development projects rely heavily on this information, it can be challenging to obtain. Most often, groups involved in process development do not have the analytical tools or resources required for this type of analysis. This means that culture samples need to be sent out to on-site or off-site core labs. Outsourcing can take days to weeks from sample-to-result and thus severely delaying process development efforts…

The Rotea Counterflow Centrifugation System Offers a Versatile, Closed System Solution for Cell Therapy Processing

Cell therapy processing requires multiple steps and procedures including cell separation, concentration, washing and others. For a successful cell therapy process, it is important to be able to conduct these activities as simply, safely and efficiently as possible. To improve the efficiency of cell processing operations, Thermo Fisher Scientific launched the multipurpose Gibco™ Cell Therapy Systems (CTS™) Rotea™ Counterflow Centrifugation System. The system uses a closed single-use kit to allow smooth transition from research through commercial manufacturing…

Xcellerex Automated Perfusion System provides a single-use, automated solution to simplify perfusion production

Perfusion culture is used to intensify bioprocess operations and provide improvement in efficiency and productivity for several different applications. Perfusion culture results in increased product output by continuously culturing and harvesting product over longer periods of time compared to traditional or fed batch methods. While extremely productive, perfusion often requires complex and labor-intensive set up and manual operation. It can also be limited in its single-use options, process integration and automation…